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BIO 45: Introduction to Laboratory Research in Cell and Molecular Biology

Investigate yeast strains that are engineered to express the human tumor suppressor protein, p53, and use modern molecular methods to identify the functional consequences of p53 mutations isolated from tumor cells. Learn about the protein's role as Guardian of the Genome through lectures and by reading and discussing journal articles. Use molecular visualization programs to examine the structure of normal and mutant p53 proteins. Learn about the assays used to study the ability of mutant p53 to direct expression of several reporter genes. During guided reflection, investigate further and identify what could be wrong with the p53 mutants you have been studying. Learn how to ask a question, test a hypothesis, conduct experiments and analyze data, and present your findings through a team oral presentation, as well as a scientific poster. Although there are no pre-requisites to enroll in this class, it will be helpful if you have already taken or are concurrently enrolled in the appropriate Biology Foundation classes (or HumBio core classes). Additionally, it will also be helpful if you have already taken CHEM 31X, or 31A & B, and 33.
Terms: Aut, Win, Sum | Units: 4 | UG Reqs: WAY-SMA
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