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BIO 132: Advanced Imaging Lab in Biophysics (APPPHYS 232, BIO 232, BIOPHYS 232, GENE 232)

Laboratory and lectures. Advanced microscopy and imaging, emphasizing hands-on experience with state-of-the-art techniques. Students construct and operate working apparatus. Topics include microscope optics, Koehler illumination, contrast-generating mechanisms (bright/dark field, fluorescence, phase contrast, differential interference contrast), and resolution limits. Laboratory topics vary by year, but include single-molecule fluorescence, fluorescence resonance energy transfer, confocal microscopy, two-photon microscopy, microendoscopy, and optical trapping. Limited enrollment. Recommended: basic physics, basic cell biology, and consent of instructor.
Terms: Spr | Units: 4 | UG Reqs: GER: DB-NatSci | Grading: Letter (ABCD/NP)
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