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BIO 152: Imaging: Biological Light Microscopy (CSB 222, MCP 222)

This intensive laboratory and discussion course will provide participants with the theoretical and practical knowledge to utilize emerging imaging technologies based on light microscopy. Topics include microscope optics, resolution limits, Köhler illumination, confocal microscopy, fluorescence, two-photon, TIRF, FRET, photobleaching, super-resolution (SIM, STED, STORM/PALM), and live-cell imaging. Applications include using fluorescent probes to analyze subcellular localization and live cell-translocation dynamics. We will be using a ¿flipped¿ classroom for the course in that students will watch iBiology lectures before class, and class time will be used for engaging in extensive discussion. Lab portion involves extensive in-class use of microscopes in the Stanford Cell Sciences Imaging Facility (CSIF) and Neuroscience Microscopy Core (NMS) microscopy facilities. nnMonday/Wednesday 1:30-2:50PM, Friday 1:30-4:30PM for 6 weeks (Apr. 2 - May 9), 3 units
Terms: Spr | Units: 3 | UG Reqs: GER: DB-NatSci | Grading: Medical Option (Med-Ltr-CR/NC)
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